PIK3CA Mutation Test qPCR Kit

PIK3CA Mutation Test qPCR Kit

The GeneFirst OncoPlex® PIK3CA Mutation Test Kit (RUO) is a real-time PCR-based test that detect 4 mutations in the human PIK3CA gene with high sensitivity. The basis for the GeneFirst proprietary technology, MMD, is a combination of specially designed mutation-specific primers, blocker-probe and unique cycling conditions that facilitate selective amplification of multiple mutant alleles – at the same time, blocking amplification from wild type (normal) allele. This allows for the detection of rare mutations against a high background of normal, non-mutated genomic DNA.